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11 record(s) found

Agripreneurs level of readiness for environmentally friendly mushroom cultivation waste management

Abstract

Mushrooms are considered as an environmentally-friendly crops by using agricultural waste as substrate, while spent mushroom substrate (SMS) is reusable. However, mushroom cultivation waste (MCW) to be managed in environmentally-friendly is not much practiced by agripreneurs that led impact to the environment. Therefore, this study attempts to evaluate the level of agripreneurs' readiness on environmentallyfriendly management in post-harvest stage. It also evaluated the method of obtaining information regarding MCW management. Survey and interview were conducted involving 40 agripreneurs who were participants of the Young Mushroom Agripreneur Project Package Course. The results showed that agripreneurs knew and aware of caring the environmental quality when dealing with MCW, but most of them did not manage in a proper way, despite received advices from agency. Expanding agripreneurs awareness, skills capabilities in post-harvest techniques, and building-up networking with agencies and .....

Article Published 2020 1580 hits Available

Keberkesanan pengajaran dan pembelajaran penulisan karangan dalam meningkatkan kualiti karangan pelajar menengah atas: satu tinjauan

Noorhayati Mushroom

Abstract

Kajian ini tentang keberkesanan pengajaran dan pembelajaran penulisan karangan dalam meningkatkan kualiti karangan pelajar menengah atas. Kajian ini menggunakan kaedah soal selidik. Seramai 233 orang pelajar dari salah sebuah sekolah di Daerah Larut Matang dan Selama dipilih sebagai responden. Empat orang guru juga dipilih bagi mengisi borang data. Pemboleh ubah bebas dalam kajian ini ialah ciri-ciri demografi, iaitu jantina, gred, masalah pelajar, latihan penulisan, aktiviti pengajaran dan pembelajaran yang dilaksanakan semasa proses pengajaran dan pembelajaran penulisan karangan. Pemboleh ubah bersandar ialah keberkesanan pengajaran dan pembelajaran penulisan karangan dalam meningkatkan kualiti karangan pelajar. Kajian ini bertujuan melihat masalah-masalah pelajar dalam pembelajaran penulisan karangan, teknik pengajaran yang berkesan dalam meningkatkan kualiti karangan pelajar dan hubungan antara keker.....

Thesis Published 2010 474 hits Available

In Vitro Pharmacological Evaluation of the Leaves and Stem Bark Extracts of Beilschmiedia penangiana Gamble

Wan Mohd Nuzul Hakimi Wan Salleh

Abstract

The leaves and stem bark extracts of B. penangiana were screened to evaluate their antioxidant, antimicrobial,tyrosinase, lipoxygenase, and acetylcholinesterase activities. Evaluation of antioxidant activities were tested against DPPH, ABTS, and total phenolic content (TPC) assays. The antimicrobial activity were investigated by microdilution method for determination of MIC, MBC, and MFC. Tyrosinase and lipoxygenase inhibitory activities were analysed using mushroom tyrosinase and lipoxygenase enzymes, respectively. In addition, the acetylcholinesterase activity was tested using cholinesterase enzyme. The methanol extract of stem bark showed the highest phenolic content (176.8 mg GAE/g), DPPH free radical scavenging (IC50 95.5 μg/mL), ABTS (IC50 124.8 μg/mL), and lipoxygenase inhibitory activity (I: 46.3%). All extracts showed from moderate to weak activity with MIC/MBC/MFC values in the ranged of 62.5-1000 µg/mL. The ethyl acetate stem bark extract revealed the highest percentage i.....

Article Published 2019 16340 hits Limited

Phytochemicals and tyrosinase inhibitory activity from piper caninum and piper magnibaccum

Wan Mohd Nuzul Hakimi Wan Salleh

Abstract

Background: Piper species are aromatic plants used as spices in the kitchen, but their secondary metabolites have also shown biological effects on human health. In traditional medicine, Piper species have been used worldwide to treat several diseases such as urological problems, skin, liver and stomach ailments, for wound healing, and as antipyretic and anti-inflammatory agents. In the present study, we attempted to isolate the phytochemicals from Piper caninum and Piper magnibaccum and evaluate their tyrosinase inhibitory activity. Methods: Phytochemical constituents of the extract were investigated using various chromatographic and spectroscopic methods. The structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme. Results: Fractionation and purification of the extracts of Piper.....

Article Published 2019 2844 hits Available

Antityrosinase inhibitory activity of phytochemicals from alpinia aquatica roscoe

Nissha Bharrathi Romes

Abstract

Background: Genus Alpinia are commonly used as spices and ingredients in traditional medicines. In the present study, we attempted to isolate the phytochemicals from Alpinia aquatica and evaluate their tyrosinase inhibitory activity. Methods: Phytochemical constituents of the extract were investigated using various chromatographic and spectroscopic methods. The chemical structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme. Results: Fractionation and purification of the extracts of Alpinia aquatica afforded seven known compounds which are 5-hydroxy-3,7,4’-trimethoxyflavone (1), 4’,5-dihydroxy-3,7-dimethoxyflavone (2), 2-methoxy-8-(2’,4’,5’-trimethoxyphenyl)-1,4-naphthaquinone (3), cis-3S-(2’,4’,5’-trimethoxyphenyl)-4S-[(E)-2’’’,4’’’,5’’’-trimet.....

Article Published 2020 889 hits Available

An in vitro cytotoxicity of a novel pH-sensitive lectin loaded-cockle shell-derived calcium carbonate nanoparticles against MCF-7 breast tumour cell

Al-Obaidi, Jameel R.

Abstract

The acidic microenvironment of tumour cells requires a pH-sensitive delivery mechanism for improving drug release. In this study, we investigated the feasibility of using pH-sensitive ACC nanoparticles with sustained-release performance and slow degradability as carriers for lectins isolated from edible mushroom Agaricus bisporus (ABL) that exhibited anti-proliferating effects on tumour cells. ABL was purified and characterized using ion-exchange, gel filtration chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. ABL-ACC nanoparticles were synthesized through a simple precipitation process coupled with mechanical grinding. The conjugated ABL-ACC-NPs were characterized using Fourier-transform infrared spectroscopy, X-ray powder diffraction, scanning electron microscope, transmission electron microscope and zeta potential. Cytotoxicity of ABL-ACC-NPs was analyzed using methylthiazol tetrazolium assay against MCF-7 cells compared with ABL and A.....

Article Published 2021 1763 hits Available

Protein profile of MCF-7 breast cancer cell line treated with lectin delivered by CaCO3NPs revealed changes in molecular chaperones, cytoskeleton, and membrane-associated proteins

Al-Obaidi, Jameel R.

Abstract

The second most predominant cancer in the world and the first among women is breast cancer. We aimed to study the protein abundance profiles induced by lectin purified from the Agaricus bisporus mushroom (ABL) and conjugated with CaCO3NPs in the MCF-7 breast cancer cell line. Two-dimensional electrophoresis (2-DE) and orbitrap mass spectrometry techniques were used to reveal the protein abundance pattern induced by lectin. Flow cytometric analysis showed the accumulation of ABL-CaCO3NPs treated cells in the G1 phase than the positive control. Thirteen proteins were found different in their abundance in breast cancer cells after 24 h exposure to lectin conjugated with CaCO3NPs. Most of the identified proteins were showing a low abundance in ABL-CaCO3NPs treated cells in comparison to the positive and negative controls, including V-set and immunoglobulin domain, serum albumin, actin cytoplasmic 1, triosephosphate isomerase, tropomyosin alpha-4 chain, and endoplasmic reticulum chaperone B.....

Article Published 2021 1275 hits Available

Essential oil composition, anti-tyrosinase activity, and molecular docking studies of Knema intermedia Warb. (Myristicaceae)

Wan Mohd Nuzul Hakimi Wan Salleh

Abstract

Knema is one of the genera in the Myristicaceae family. The genus includes 60 species in Southeast Asia and is traditionally used for treating skin disorders. Here, for the first time, the essential oil, anti-tyrosinase, and molecular docking studies of Knema intermedia were evaluated. The essential oil was obtained by hydrodistillation and fully characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Anti-tyrosinase activity was evaluated against mushroom tyrosinase, whereas molecular docking studies were performed using Autodock vina embedded in PyRx to evaluate the binding interactions of major components. A total of 37 components (97.3%) were successfully identified in the essential oil, which was characterized by high amounts of t-muurolol (20.1%), ?-copaene (14.4%), ?-cadinene (13.9%), germacrene B (9.5%), and ?-selinene (7.0%). The essential oil displayed moderate inhibitory activity towards tyrosinase with an IC50 value of 70.2 ?g/mL. The .....

Article Published 2023 428 hits Available

Chemical composition, anti-tyrosinase activity and molecular docking studies of knema malayana warb. essential oil

Wan Mohd Nuzul Hakimi Wan Salleh

Abstract

Essential oils produced from aromatic plants denote significant biological activities and are gaining prominence in scientific research. In this study, the chemical composition, anti-tyrosinase activity, and molecular docking studies of Knema malayana Warb. essential oil was investigated. A hydrodistillation method was used to produce the essential oil, characterised by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC/MS). Additionally, anti-tyrosinase activity was evaluated against mushroom tyrosinase, while molecular docking studies were performed with the major components. A total of 38 components (98.9%) were successfully identified in the essential oil, which was characterised by high proportions of ?-cadinene (20.2%), ?-cadinol (13.7%), ?-amorphene (10.3%), ?-cadinene (6.3%), ?-gurjunene (5.3%), ?-gurjunene (5.3%), and ?-muurolene (5.0%). The essential oil demonstrated moderate activity towards tyrosinase activity with an IC50 value of 85.3 ?g/mL. Conseque.....

Article Published 2023 353 hits Available

Chemical composition, anti-tyrosinase, and molecular docking studies of Knema furfuracea Warb. essential oil

Wan Mohd Nuzul Hakimi Wan Salleh

Abstract

Knema furfuracea Warb., a tree species belonging to the family Myristicaceae and indigenous to Southeast Asia, has been traditionally used by the Dai people for the treatment of inflammation and other illnesses. This work investigates the chemical composition of the essential oil of K. furfuracea, antityrosinase activity, and molecular docking studies. The essential oil was obtained through hydrodistillation and characterised through gas chromatography (GC/FID) and gas chromatography mass spectrometry (GC/ MS). The anti-tyrosinase activity was determined using mushroom tyrosinase enzyme. The major components of the essential oil were studied for their potential interactions with tyrosinase using Autodock vina. A total of thirty-one components (96.0%) were identified from the leaf oil and composed mainly of bicyclogermacrene (23.1%), δ-cadinene (17.2%), (E,E)-α-farnesene (9.1%), and β-caryophyllene (7.7%). The essential oil demonstrated moderate activity towards tyrosinase with an IC.....

Article Published 2024 200 hits Available

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