AbstractThis study aimed to produce allergen extracts, to identify the protein profiles, frequency of allergy, major and minor allergens, and subsequently to determine the effect of heat treatments on allergenicity of two species of local edible snails, Cerithidea obtusa (C. obtusa) and Pomacea canaliculata (P. canaliculata). C. obtusa samples were obtained from Perak, Melaka and Selangor while P. canaliculata samples were obtained from Kelantan, Melaka and Penang. Proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 131 patients with atopic diseases in Kuala Lumpur Hospital were skin prick tested (SPT) with raw snail extracts. Immunoblotting of raw and heated extracts was performed using sera from patients with snail allergy. Through SDS-PAGE, raw extract of C. obtusa was found to contain 32 protein bands, between 11 to 250 kDa while P. canaliculata had only 31 prot.....