AbstractThe purpose of this study was to assess and analyse the Pedagogical Content Knowledge (PCK) of novice and experienced Biology teachers. The study examined five components namely, content knowledge, pedagogical knowledge, knowledge of student’s, teacher’s beliefs in the nature of Biology and teaching of Biology. It also aimed to investigate the integration of these components in producing an effective teaching of Biology. Respondents of the study comprised four teachers involving two novice teachers (1-3 years teaching experience) and two experienced teachers (more than 5 years of teaching experience) who were teaching Biology at secondary schools in the district of Rompin, Pahang. This study employed the case study design. The data of this study were analysed thematically with information gathered through semi-structured interview, non-involvement of distant observation and document analysis. This study found that experienced teachers are able to balance PCK components. Experienced.....