The isolation and purification of the leaves of Kopsia singapurensisRidl. (Apocynaceae) revealed the presence of steroids, terpenes, and alkaloids. Stigmasterol 1 was isolated from the hexane crude extract of the leaves of this species. This phytochemical study involves extraction, separation by using various chromatographic methods and structural determination by spectroscopic technique such as IR, NMR including 1D-NMR (1H, 13C and DEPT), 2D-NMR (COSY, NOESY, HMQC/HSQC, and HMBC) and GCMS, involving also comparison with data from the literature..

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In Malaysia, Alstonia spathulata Bl. is locally known as ‘Pulai Basong’ which distribute in peat forest. The separation of the chemical components from hexane and DCM crude extract of Alstonia spathulata was carried out using column chromatography (CC) and thin layer chromatography (TCL) techniques. A total of five known compound were isolated from the stem bark extracts and identified as: β-amyrin, β-amyrin acetate, lupeol acetate, stigmasterol and β-sitosterol. The elucidation structures of these compounds were established using 1D-NMR (1H, 13C and DEPT) and 2D-NMR (COSY, HSQC, HMBC) spectroscopic analysis, involving also comparison with data from the literature...

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